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Hookworm

This article is about the organism. For the infection by Necator americanus, see Necatoriasis. For the infection by Ancylostoma duodenale, see Ancylostomiasis. For the British rock band, see Hookworms (band).

Hookworm is a parasitic worm that lives in the small intestine of its host, which may be a mammal such as a dog, cat, or human. In children hookworms cause intellectual, cognitive and growth retardation, intrauterine growth retardation, prematurity, and low birth weight among newborns born to infected mothers.

Hookworm
Classification and external resources
ICD-10Template:ICD10
ICD-9Template:ICD9

Three species of hookworms commonly infect humans: Ancylostoma duodenale, Necator americanus and Strongyloides stercoralis. A. duodenale predominates in the Middle East, North Africa, India and (formerly) in southern Europe, while N. americanus predominates in the Americas, Sub-Saharan Africa, Southeast Asia, China, and Indonesia. The A. braziliense and A. tubaeforme species infect cats, while A. caninum infects canines. Uncinaria stenocephala infects both dogs and cats. Hookworms are much smaller than the giant roundworm (Ascaris lumbricoides), and the complications of tissue migration and mechanical obstruction so frequently observed with roundworm infestation are less frequent in hookworm infestation. The most significant risk of hookworm infection is anemia, secondary to loss of iron (and protein) in the gut. The worms suck blood voraciously and damage the mucosa. However, the blood loss in the stools is not visibly apparent.

Hookworm affects over half a billion people globally.[1] It is a leading cause of maternal and child morbidity in the developing countries of the tropics and subtropics. In developed countries, hookworm infection is rarely fatal, but anemia can be significant in a heavily infected individual. Hookworm infection belong to the group of soil-transmitted helminthiasis and is as such classified as a neglected tropical disease.[2] Ancylostomiasis, also known by several other names, is the disease caused when A. duodenale hookworms, present in large numbers, produce an iron deficiency anemia by sucking blood from the host's intestinal walls.

Signs and symptoms

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There are no specific symptoms or signs of hookworm infection. As mentioned above, they give rise to a combination of intestinal inflammation and progressive iron/protein-deficiency anemia. Larval invasion of the skin might give rise to intense, local itching, usually on the foot or lower leg, which can be followed by lesions that look like insect bites, can blister ("ground itch"), and last for a week or more.

Animal hookworm larvae on penetrating humans may produce a creeping eruption called cutaneous larva migrans. The larvae migrate in tortuous tunnels in between stratum basale and stratum corneum of the skin, causing serpigenous vesicular lesions. With advancing movement of the larvae, the rear portions of the lesions become dry and crusty. The lesions are typically intensely itchy.

Coughing, chest pain, wheezing, and fever will sometimes be experienced by people who have been exposed to very large numbers of larvae. Epigastric pains, indigestion, nausea, vomiting, constipation, and diarrhea can occur early or in later stages as well, although gastrointestinal symptoms tend to improve with time. Signs of advanced severe infection are those of anemia and protein deficiency, including emaciation, cardiac failure and abdominal distension with ascites.

Pathophysiology

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Morphology

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A. duodenale worms are grayish white or pinkish with the head slightly bent in relation to the rest of the body. This bend forms a definitive hook shape at the anterior end for which hookworms are named. They possess well developed mouths with two pairs of teeth. While males measure approximately one centimeter by 0.5 millimeter, the females are often longer and stouter. Additionally, males can be distinguished from females based on the presence of a prominent posterior copulatory bursa.[3]

N. americanus is very similar in morphology to A. duodenale. N. americanus is generally smaller than A. duodenale with males usually 5 to 9 mm long and females about 1 cm long. Whereas A. duodenale possess two pairs of teeth, N. americanus possesses a pair of cutting plates in the buccal capsule. Additionally, the hook shape is much more defined in Necator than in Ancylostoma.[3]

Pathology

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Hookworm infection is generally considered to be asymptomatic, but as Norman Stoll described in 1962, hookworm is an extremely dangerous infection because its damage is “silent and insidious.”[4] There are general symptoms that an individual may experience soon after infection. Ground-itch, which is an allergic reaction at the site of parasitic penetration and entry, is common in patients infected with N. americanus.[3] Additionally, cough and pneumonitis may result as the larvae begin to break into the alveoli and travel up the trachea. Then once the larvae reach the small intestine of the host and begin to mature, the infected individual will suffer from diarrhea and other gastrointestinal discomfort.[3] However, the “silent and insidious” symptoms referred to by Stoll are related to chronic, heavy-intensity hookworm infections. Major morbidity associated with hookworm is caused by intestinal blood loss, iron deficiency anemia, and protein malnutrition.[5] They result mainly from adult hookworms in the small intestine ingesting blood, rupturing erythrocytes, and degrading hemoglobin in the host.[6] This long-term blood loss can manifest itself physically through facial and peripheral edema; eosinophilia and pica caused by iron deficiency anemia are also experienced by some hookworm-infected patients.[3] Recently, more attention has been given to other important outcomes of hookworm infection that play a large role in public health. It is now widely accepted that children who suffer from chronic hookworm infection can suffer from growth retardation as well as intellectual and cognitive impairments.[6][7] Additionally, recent research has focused on the potential of adverse maternal-fetal outcomes when the mother is infected with hookworm during pregnancy.

The disease was linked to nematode worms (Ankylostoma duodenalis) from one-third to half an inch long in the intestine chiefly through the labours of Theodor Bilharz and Griesinger in Egypt (1854).

The symptoms can be linked to inflammation in the gut stimulated by feeding hookworms, such as nausea, abdominal pain and intermittent diarrhea, and to progressive anemia in prolonged disease: capricious appetite, pica (or dirt-eating), obstinate constipation followed by diarrhea, palpitations, thready pulse, coldness of the skin, pallor of the mucous membranes, fatigue and weakness, shortness of breath and in cases running a fatal course, dysentery, hemorrhages and edema.

Blood tests in early infection often show a rise in numbers of eosinophils, a type of white blood cell that is preferentially stimulated by worm infections in tissues (large numbers of eosinophils are also present in the local inflammatory response). Falling blood hemoglobin levels will be seen in cases of prolonged infection with anemia.

In contrast to most intestinal helminthiases, where the heaviest parasitic loads tend to occur in children, hookworm prevalence and intensity can be higher among adult males. The explanation for this is that hookworm infection tends to be occupational, so that coworkers and other close groups maintain a high prevalence of infection among themselves by contaminating their work environment. However, in most endemic areas, adult women are the most severely affected by anemia, mainly because they have much higher physiological needs for iron (menstruation, repeated pregnancy).

An interesting consequence of this in the case of Ancylostoma duodenale infection is translactational transmission of infection: the skin-invasive larvae of this species do not all immediately pass through the lungs and on into the gut, but spread around the body via the circulation, to become dormant inside muscle fibers. In a pregnant woman, after childbirth some or all of these larvae are stimulated to re-enter the circulation (presumably by sudden hormonal changes), then to pass into the mammary glands, so that the newborn baby can receive a large dose of infective larvae through its mother's milk. This accounts for otherwise inexplicable cases of very heavy, even fatal, hookworm infections in children a month or so of age, in places such as China, India and northern Australia.

An identical phenomenon is much more commonly seen with Ancylostoma caninum infections in dogs, where the newborn pups can even die of hemorrhaging from their intestines caused by massive numbers of feeding hookworms. This also reflects the close evolutionary link between the human and canine parasites, which probably have a common ancestor dating back to when humans and dogs first started living closely together. Filariform larvae is the infective stage of the parasite, infection occurs when larvae in soil prentrate the skin or when they are ingested through contaminated food and water following skin penetration, larvae

Life cycle

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Hookworm life cycle

See the image for the biological life cycle of the hookworm where it thrives in warm earth where temperatures are over 18 °C. They exist primarily in sandy or loamy soil and cannot live in clay or muck. Rainfall averages must be more than 1000 mm (40 inches) a year for them to survive. Only if these conditions exist can the eggs hatch. Infective larvae of Necator americanus can survive at higher temperatures, whereas those of Ancylostoma duodenale are better adapted to cooler climates. Generally, they live for only a few weeks at most under natural conditions, and die almost immediately on exposure to direct sunlight or desiccation.

Infection of the host is by the larvae, not the eggs. While A. duodenale can be ingested, the usual method of infection is through the skin; this is commonly caused by walking barefoot through areas contaminated with fecal matter. The larvae are able to penetrate the skin of the foot, and once inside the body, they migrate through the vascular system to the lungs, and from there up the trachea, and are swallowed. They then pass down the esophagus and enter the digestive system, finishing their journey in the intestine, where the larvae mature into adult worms.[6][8]

Once in the host gut, Necator tends to cause a prolonged infection, generally 1–5 years (many die within a year or two of infecting), though some adult worms have been recorded to live for 15 years or more. On the other hand, Ancylostoma adults are short lived, surviving on average for only about 6 months. However, infection can be prolonged because dormant larvae can be "recruited" sequentially from tissue "stores" (see Pathology, above) over many years, to replace expired adult worms. This can give rise to seasonal fluctuations in infection prevalence and intensity (apart from normal seasonal variations in transmission).

 
Civilian Public Service workers built and installed 2065 outhouses for hookworm eradication in Mississippi and Florida from 1943 to 1947.

They mate inside the host, females laying up to 30,000 eggs per day and some 18 to 54 million eggs during their lifetime, which pass out in feces. Because it takes 5–7 weeks for adult worms to mature, mate and produce eggs, in the early stages of very heavy infection, acute symptoms might occur without any eggs being detected in the patient's feces. This can make diagnosis very difficult.

Summary of biological life cycle

N. americanus and A. duodenale eggs can be found in warm, moist soil where they will eventually hatch into first stage larvae, or L1. L1, the feeding non-infective rhabditoform stage, will feed on soil microbes and eventually molt into second stage larvae, L2. L2, which is also in the rhabditoform stage, will feed for approximately 7 days and then molt into the third stage larvae, or L3. L3 is the filariform stage of the parasite, that is, the non-feeding infective form of the larvae. The L3 larvae are extremely motile and will seek higher ground to increase their chances of penetrating the skin of a human host. The L3 larvae can survive up to 2 weeks without finding a host. While N. americanus larvae only infect through penetration of skin, A. duodenale can infect both through penetration as well as orally. After the L3 larvae have successfully entered the host, the larvae then travel through the subcutaneous venules and lymphatic vessels of the human host. Eventually, the L3 larvae enter the lungs through the pulmonary capillaries and break out into the alveoli. They will then travel up the trachea to be coughed and swallowed by the host. After being swallowed, the L3 larvae are then found in the small intestine where they molt into the L4, or adult worm stage. The entire process from skin penetration to adult development takes about 5–9 weeks. The female adult worms will release eggs (N. americanus about 9,000–10,000 eggs/day and A. duodenale 25,000–30,000 eggs/day) which are passed in the feces of the human host. These eggs will hatch in the environment within several days and the cycle will start anew.[5][6][9]

Incubation period

The incubation period can vary between a few weeks to many months and is largely dependent on the number of Hookworm parasites an individual is infected with.[10]

Diagnosis

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Hookworm egg

Diagnosis depends on finding characteristic worm eggs on microscopic examination of the stools, although this is not possible in early infection. Early signs of infection in most dogs include limbular limping and anal itching. The eggs are oval or elliptical, measuring 60 µm by 40 µm, colourless, not bile stained and with a thin transparent hyaline shell membrane. When released by the worm in the intestine, the egg contains an unsegmented ovum. During its passage down the intestine, the ovum develops and thus the eggs passed in feces have a segmented ovum, usually with 4 to 8 blastomeres. As the eggs of both Ancylostoma and Necator (and most other hookworm species) are indistinguishable, to identify the genus, they must be cultured in the lab to allow larvae to hatch out. If the fecal sample is left for a day or more under tropical conditions, the larvae will have hatched out, so eggs might no longer be evident. In such a case, it is essential to distinguish hookworms from Strongyloides larvae, as infection with the latter has more serious implications and requires different management. The larvae of the two hookworm species can also be distinguished microscopically, although this would not be done routinely, but usually for research purposes. Adult worms are rarely seen (except via endoscopy, surgery or autopsy), but if found, would allow definitive identification of the species. Classification can be performed based on the length of the buccal cavity, the space between the oral opening and the esophagus: hookworm rhabditoform larvae have long buccal cavities whereas Strongyloides rhabditoform larvae have short buccal cavities.[3]

Recent research has focused on the development of DNA-based tools for diagnosis of infection, specific identification of hookworm, and analysis of genetic variability within hookworm populations.[11] Because hookworm eggs are often indistinguishable from other parasitic eggs, PCR assays could serve as a molecular approach for accurate diagnosis of hookworm in the feces.[11][12]

Prevention

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The infective larvae develop and survive in an environment of damp dirt, particularly sandy and loamy soil. They cannot survive in clay or muck. The main lines of precaution are those dictated by sanitary science:

  • Do not defecate in places other than latrines, toilets etc.
  • Do not use human excrement or raw sewage or untreated 'night soil' as manure or fertilizer in agriculture
  • Do not walk barefoot in known infected areas
  • Deworm pet dogs — canine and feline hookworms rarely develop to adulthood in humans (Ancylostoma caninum, the common dog hookworm, occasionally develops into an adult to cause eosinophilic enteritis in people), but their invasive larvae can cause an itchy rash called cutaneous larva migrans.

Moxidectin has been released in the United States as part of Advantage Multi (imidacloprid + moxidectin) Topical Solution for dogs and cats. It utilizes moxidectin for control and prevention of roundworms, hookworms, heartworms, and whipworms.

 
Ethiopian children treated for schistosoma and hookworms.

While hookworm infection may not directly lead to mortality, its effects on morbidity demand immediate attention. When considering disability-adjusted life years (DALYs), neglected tropical diseases, including hookworm, rank among diarrheal diseases, ischemic heart disease, malaria, and tuberculosis as one of the most important health problems of the developing world.

It has been estimated that as many as 22.1 million DALYs have been lost due to hookworm. Recently, there has been increasing interest to address the public health concerns associated with hookworm. For example, the Bill & Melinda Gates Foundation recently donated US$34 million to fight Neglected Tropical Diseases including hookworm infection.[13] Former US President Clinton also announced a mega-commitment at the Clinton Global Initiative (CGI) 2008 Annual Meeting to de-worm 10 million children.[14]

Most of these public health concerns have focused on children who are infected with hookworm. This focus on children is largely due to the large body of evidence that has demonstrated strong associations between hookworm infection and impaired learning, increased absences from school, and decreased future economic productivity.[6] In 2001, the 54th World Health Assembly passed a resolution demanding member states to attain a minimum target of regular deworming of at least 75% of all at-risk school children by the year 2010.[15] A 2008 World Health Organization publication reported on these efforts to treat at-risk school children. Some of the interesting statistics were as follows: 1) only 9 out of 130 endemic countries were able to reach the 75% target goal; and 2) less than 77 million school-aged children (of the total 878 million at risk) were reached which means that only 8.78% of at-risk children are being treated for hookworm infection.[16] While there is progress being made, these numbers also remind us of how much work is still to be done.

School-based mass deworming programs have been the most popular strategy to address the issue of hookworm infection in children. School-based programs are extremely cost effective as schools already have an available, extensive, and sustained infrastructure with a skilled workforce that has a close relationship with the community.[15] With little training from a local health system, teachers can easily administer the drugs which often cost less than US$0.50 per child per year.[17]

Recently, many people have begun to question if the school-based programs are necessarily the most effective approach. An important concern with school-based programs is that they often do not reach children who do not attend school, thus ignoring a large amount of at-risk children. A 2008 study by Massa et al. continued the debate regarding school-based programs. They examined the effects of community-directed treatments versus school-based treatments in the Tanga Region of Tanzania. A major conclusion was that the mean infection intensity of hookworm was significantly lower in the villages employing the community-directed treatment approach than the school-based approach. The community-directed treatment model used in this specific study allowed villagers to take control of the child's treatment by having villagers select their own community drug distributors to administer the antihelminthic drugs. Additionally, villagers organized and implemented their own methods for distributing the drugs to all children.[18] The positive results associated with this new model highlight the need for large-scale community involvement in deworming campaigns.

Many mass deworming programs also combine their efforts with a public health education. These health education programs often stress important preventative techniques such as: washing your hands before eating, and staying away from water/areas contaminated by human faeces. These programs may also stress that shoes must be worn, however these come with their own health risks and may not be effective[19] Shoe wearing patterns in towns and villages across the globe are determined by cultural beliefs, and the levels of education within that society. The wearing of shoes will prevent the entry of hookworm infections from the surrounding soils into tender skin regions; such as areas between the toes[20]

Historical examples, such as the hookworm campaigns in Mississippi and Florida from 1943 to 1947 have shown that the primary cause of Hookworm is poor sanitation, which can be solved by building and maintaining outhouses. But while these may seem like simple tasks, they raise important public health challenges. Most infected populations are from poverty-stricken areas with very poor sanitation. Thus, it is most likely that at-risk children do not have access to clean water to wash their hands and live in environments with no proper sanitation infrastructure. Health education, therefore, must address preventive measures in ways that are both feasible and sustainable in the context of resource-limited settings.

Evaluation of numerous public health interventions have generally shown that improvement in each individual component ordinarily attributed to poverty (for example, sanitation, health education and underlying nutrition status) often have minimal impact on transmission. For example, one study found that the introduction of latrines into a resource-limited community only reduced the prevalence of hookworm by four percent.[21] However, another study in Salvador, Brazil found that improved drainage and sewerage had a significant impact (p<0.0001) on the prevalence of hookworm but no impact at all on the intensity of hookworm.[22] This seems to suggest that environmental control alone has a limited but incomplete effect on the transmission of hookworm. It is imperative, therefore, that more research be performed to understand the efficacy and sustainability of integrated programs that combine numerous preventive methods including education, sanitation, and treatment.

Management

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The most common treatment for hookworm are benzimidazoles, specifically albendazole and mebendazole. BZAs kill adult worms by binding to the nematode’s β-tubulin and subsequently inhibiting microtubule polymerization within the parasite.[5] In certain circumstances, levamisole and pyrantel pamoate may be used.[6] The 2008 study by Keiser and Utzinger, Efficacy of Current Drugs Against Soil-Transmitted Helminth Infections: Systematic Review and Meta-analysis, examined the relative efficacies of different drug treatments. They found that the efficacy of single-dose treatments for Hookworm infections were as follows: 72% for albendazole, 15% for mebendazole, and 31% for pyrantel pamoate.[23] This substantiates prior claims that albendazole is much more effective than mebendazole for Hookworm infections. Also of note is that the World Health Organization does recommend anthelmintic treatment in pregnant women after the first trimester.[5] It is also recommended that if the patient also suffers from anemia that ferrous sulfate (200 mg) be administered three times daily at the same time as anthelmintic treatment; this should be continued until hemoglobin values return to normal which could take up to 3 months.[3]

Other important issues related to the treatment of hookworm are reinfection and drug resistance. It has been shown that reinfection after treatment can be extremely high. Some studies even show that 80% of pretreatment hookworm infection rates can be seen in treated communities within 30–36 months.[5] While reinfection may occur, it is still recommended that regular treatments be conducted as it will minimize the occurrence of chronic outcomes. There are also increasing concerns about the issue of drug resistance. Drug resistance has appeared in front-line anthelmintics used for livestock nematodes. Generally human nematodes are less likely to develop resistance due to longer reproducing times, less frequent treatment, and more targeted treatment. Nonetheless, the global community must be careful to maintain the effectiveness of current anthelmintic as no new anthelmintic drugs are in the late-stage development.[5]

The hookworm can be treated with local cryotherapy when it is still in the skin.[24]

Albendazole is effective both in the intestinal stage and during the stage the parasite is still migrating under the skin.[24]

In case of anemia, iron supplementation can cause relief symptoms of iron deficiency anemia. However, as red blood cell levels are restored, shortage of other essentials such as folic acid or vitamin B12 may develop, so these might also be supplemented.

Epidemiology

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Disability-adjusted life year for hookworm disease per 100,000 inhabitants in 2002.
  no data
  less than 10
  10–15
  15–20
  20–25
  25–30
  30–35
  35–40
  40–45
  45–50
  50–55
  55–60
  more than 60

It is estimated that between 576-740 million individuals are infected with hookworm.[1][5] Of these infected individuals, about 80 million are severely affected.[11] The major etiology of hookworm infection is N. americanus which is found the Americas, sub-Saharan Africa, and Asia.[6] A. duodenale is found in more scattered focal environments, namely Europe and the Mediterranean. Most infected individuals are concentrated in sub-Saharan Africa and East Asia/the Pacific Islands with each region having estimates of 198 million and 149 million infected individuals, respectively. Other affected regions include: South Asia (50 million), Latin America and the Caribbean (50 million), South Asia (59 million), Middle East/North Africa (10 million).[5] A majority of these infected individuals live in poverty-stricken areas with poor sanitation. Hookworm infection is most concentrated among the world’s poorest who live on less than $2 a day.[6]

Many of the numbers regarding the prevalence of hookworm infection are estimates as there is no international surveillance mechanism currently in place to determine prevalence and global distribution.[6] Some prevalence rates have been measured through survey data in endemic regions around the world. The following are some of the most recent findings on prevalence rates in regions endemic with hookworm.

Darjeeling, Hooghly District, West Bengal, India (Pal et al. 2007)[25]

  • 42.8% infection rate of predominantly N. americanus although with some A. duodenale infection
  • Both hookworm infection load and degree of anemia in the mild range

Xiulongkan Village, Hainan Province, China (Gandhi et al. 2001)[26]

  • 60% infection rate of predominantly N. americanus
  • Important trends noted were that prevalence increased with age (plateau of about 41 years) and women had higher prevalence rates than men

Hoa Binh, Northwest Vietnam (Verle et al. 2003)[27]

  • 52% of a total of 526 tested households infected
  • Could not identify species, but previous studies in North Vietnam reported N. americanus in more than 95% of hookworm larvae

Minas Gerais, Brazil (Fleming et al. 2006)[28]

  • 62.8% infection rate of predominantly N. americanus

KwaZulu-Natal, South Africa (Mabaso et al. 2004)[29]

  • Inland areas had a prevalence rate of 9.3% of N. americanus
  • Coastal plain areas had a prevalence rate of 62.5% of N. americanus

There have also been recent technological developments that will hopefully facilitate more accurate mapping of hookworm prevalence. Some researchers have begun to use geographical information systems (GIS) and remote sensing (RS) to examine helminth ecology and epidemiology. Brooker et al. utilized this technology to create helminth distribution maps of sub-Saharan Africa. By relating satellite derived environmental data with prevalence data from school-based surveys, they were able to create detailed prevalence maps. The study focused on a wide range of helminths, but interesting conclusions about hookworm specifically were found. As compared to other helminths, hookworm is able to survive in much hotter conditions and was highly prevalent throughout the upper end of the thermal range. Hopefully this information along with more detailed prevalence maps can lead to more effective public health measures.[30]

Improved molecular diagnostic tools are another technological advancement that could help improve existing prevalence statistics. Recent research has focused on the development of DNA-based tool that can be used for diagnosis of infection, specific identification of hookworm, and analysis of genetic variability in hookworm populations. Again this can serve as a major tool for different public health measures against hookworm infection. Most research regarding diagnostic tools is now focused on the creation of a rapid and cost-effective assay for the specific diagnosis of hookworm infection. Many are hopeful that its development can be achieved within the next 5 years.[11]

History

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Discovery

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The symptoms now attributed to hookworm appear in papyrus papers of ancient Egypt (c. 1500 BC), described as a derangement characterized by anemia. Avicenna, a Persian physician of the 11th century, discovered the worm in several of his patients and related it to their disease. In later times, the condition was noticeably prevalent in the mining industry in England, France, Germany, Belgium, North Queensland, and elsewhere.

Italian physician Angelo Dubini was the modern-day discoverer of the worm in 1838 after an autopsy of a peasant woman. Dubini published details in 1843 and identified the species as A. duodenale. Working in the Egyptian medical system in 1852 German physician Theodor Bilharz, drawing upon the work of colleague Wilhelm Griesinger, found these worms during autopsies and went a step further in linking them to local endemic occurrences of chlorosis, which would probably be called iron deficiency anemia today.

A breakthrough came 25 years later following a diarrhea and anemia epidemic that took place among Italian workmen employed on the Gotthard Rail Tunnel. In an 1880 paper, physicians Camillo Bozzolo, Edoardo Perroncito, and Luigi Pagliani correctly hypothesized that hookworm was linked to the fact that workers had to defecate inside the 15 km tunnel, and that many wore worn-out shoes.[31] In 1897, it was established that the skin was the principal avenue of infection and the biological life cycle of the hookworm was clarified.

Eradication programmes

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In 1899, American zoologist Charles Wardell Stiles brought this evidence to bear on health issues in the southeast United States, identifying "progressive pernicious anemia" seen in the southern United States was caused by A. duodenale and he also identified the other important hookworm species: U. Necator. Testing in the 1900s revealed very heavy infestations in school-age children. In Puerto Rico, Dr. Bailey K. Ashford, an U.S. Army physician, organized and conducted a parasite treatment campaign, which cured approximately 300,000 persons (one-third of the Puerto Rico population) and reduced the death rate from this anemia by 90 percent during the years 1903–1904.

 
A doctor examines a boy for signs of hookworm in Coffee County, Alabama, 1939.

On October 26, 1909 the Rockefeller Sanitary Commission for the Eradication of Hookworm Disease was organized as a result of a gift of US$1 million from John D. Rockefeller, Sr. The five-year program was a remarkable success and a great contribution to United States public health, instilling public education, medication, field work and modern government health departments in eleven southern states.[32] The hookworm exhibit was a prominent part of the 1910 Mississippi state fair.

The Commission found that an average of 40% of school aged children were infected with hookworm. Areas with higher levels of hookworm infection prior to the eradication programme experienced greater increases in school enrollment, attendance and literacy after the intervention. Econometric studies have shown that this effect cannot be explained by a variety of alternative factors, including differential trends across areas, changing crop prices, shifts in certain educational and health policies and the effect of malaria eradication.[33] No significant contemporaneous results were found for adults who should have benefited less from the intervention owing to their substantially lower (prior) infection rates. The program nearly eradicated hookworm and would flourish afterwards with new funding as the Rockefeller Foundation International Health Division. The RF's hookworm campaign in Mexico showed how science and politics play a role in developing health policies. It brought together government officials, health officials, public health workers, Rockefeller officials and the community. This campaign was launched to eradicate hookworms in Mexico. Although the campaign did not focus on long-term treatments, it did set the terms of the relationship between Mexico and the Rockefeller Foundation. The scientific knowledge behind this campaign helped shaped public health policies, improved public health and built a strong relationship between USA and Mexico.[34]

In the 1920s, hookworm eradication reached the Caribbean and Latin America, where great mortality was reported among Black people in the West Indies towards the end of the 18th century, as well as through descriptions sent from Brazil and various other tropical and sub-tropical regions.

Treatments

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Early treatment relied on the use of Epsom salt to reduce protective mucus, followed by thymol to kill the worms.[35] Later tetrachloroethylene was the leading method. It was not until later in the mid-20th century when new organic drug compounds were developed.

Research

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Anemia in pregnancy

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It is estimated that a third of all pregnant women in developing countries are infected with hookworm, 56% of all pregnant women in developing countries suffer from anemia, 20% of all maternal deaths are either directly or indirectly related to anemia. Numbers like this have led to an increased interest in the topic of hookworm-related anemia during pregnancy.[36] With the understanding that chronic Hookworm infection can often lead to anemia, many people are now questioning if the treatment of hookworm could effect change in severe anemia rates and thus also on maternal and child health as well. Most evidence suggests that the contribution of hookworm to maternal anemia merits that all women of child-bearing age living in endemic areas be subject to periodic anthelmintic treatment. The World Health Organization even recommends that infected pregnant women be treated after their first trimester.[5] Regardless of these suggestions, only Madagascar, Nepal and Sri Lanka have added deworming to their antenatal care programs.[37]

This lack of deworming of pregnant women is explained by the fact that most individuals still fear that anthelmintic treatment will result in adverse birth outcomes. But a 2006 study by Gyorkos et al. found that when comparing a group of pregnant women treated with mebendazole with a control placebo group, both illustrated rather similar rates in adverse birth outcomes. The treated group demonstrated 5.6% adverse birth outcomes, while the control group had 6.25% adverse birth outcomes.[36] Furthermore, Larocque et al. illustrated that treatment for hookworm infection actually led to positive health results in the infant. This study concluded that treatment with mebendazole plus iron supplements during antenatal care significantly reduced the proportion of very low birth weight infants when compared to a placebo control group.[38] Studies so far have validated recommendations to treat infected pregnant women for hookworm infection during pregnancy.

The intensity of hookworm infection as well as the species of hookworm have yet to be studied as they relate to hookworm-related anemia during pregnancy. Additionally, more research must be done in different regions of the world to see if trends noted in completed studies persist.

Malaria co-infection

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Co-infection with hookworm and Plasmodium falciparum is common in Africa.[39] Although exact numbers are unknown, preliminary analyses estimate that as many as a quarter of African schoolchildren (17.8–32.1 million children aged 5–14 years) may be coincidentally at-risk of both P. falciparum and hookworm.[40] While original hypotheses stated that co-infection with multiple parasites would impair the host’s immune response to a single parasite and increase susceptibility to clinical disease, studies have yielded contrasting results. For example, one study in Senegal showed that the risk of clinical malaria infection was increased in helminth-infected children in comparison to helminth-free children while other studies have failed to reproduce such results,[41] and even among laboratory mouse experiments the effect of helminths on malaria is variable.[42] Some hypotheses and studies suggest that helminth infections may protect against cerebral malaria due to the possible modulation of pro-inflammatory and anti-inflammatory cytokines responses.[43] Furthermore, the mechanisms underlying this supposed increased susceptibility to disease are unknown. For example, helminth infections cause potent and highly polarized immune response characterized by increased T-helper cell type 2 (Th2) cytokine and Immunoglobulin E(IgE) production.[44] However, the effect of such responses on the human immune response is unknown. Additionally, both malaria and helminth infection can cause anemia, but the effect of co-infection and possible enhancement of anemia is poorly understood.[citation needed]

Hygiene hypothesis

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The hygiene hypothesis states that infants and children who lack exposure to infectious agents are more susceptible to allergic diseases via modulation of immune system development. As Mary Ruebush writes in her book Why Dirt is Good, “what a child is doing when he puts things in his mouth is allowing his immune response to explore his environment. Not only does this allow for ‘practice’ of immune responses, which will be necessary for protection, but it also plays a critical role in teaching the immature immune response what is best ignored.[45]” The theory was first proposed by David P. Strachan who noted that hay fever and eczema were less common in children who belonged to large families.[46] Since then, studies have noted the effect of gastrointestinal worms on the development of allergies in the developing world. For example, a study in Gambia found that eradication of worms in some villages led to increased skin reactions to allergies among children.[47]

Although the exact mechanism is unknown, scientists hypothesize that the helper T cells are key players. Allergic diseases, which are immunological responses to normally harmless antigens, are driven by a TH2-mediated immune response. Bacteria, viruses, and parasites, on the other hand, elicit a TH1-mediated immune response which inhibits or down-regulates the TH2 response.[48] TH1 also inhibits the activity of TH17 which is heightened in numerous inflammatory diseases including multiple sclerosis and asthma.[49] More research is currently being performed to better understand the possible mechanism for the hygiene hypothesis.

Vaccines

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Main article: Hookworm vaccine

While annual or semi-annual mass antihelminthic administration is a critical aspect of any public health intervention, many have begun to realize how unsustainable it is due to aspects such as poverty, high rates of re-infection, and diminished efficacy of drugs with repeated use. Current research, therefore, has focused on the development of a vaccine that could be integrated into existing control programs. The goal of vaccine development is not necessarily to create a vaccine with sterilizing immunity or complete protection against immunity. A vaccine that reduces the likelihood of vaccinated individuals developing severe infections and thus reduced blood and nutrient levels could still have a significant impact on the high burden of disease throughout the world.

Current research focuses on targeting two stages in the development of the worm: the larval stage and the adult stage. Research on larval antigens has focused on proteins that are members of the pathogenesis-related protein superfamily, Ancylostoma Secreted Proteins.[50] Although they were first described in Anyclostoma, these proteins have also been successfully isolated from the secreted product of N. americanus. N. americanus ASP-2 (Na-ASP-2) is currently the leading larval-stage hookworm vaccine candidate. A randomized, double-blind, placebo-controlled study has already been performed; 36 healthy adults without a history of hookworm infection were given three intramuscular injections of three different concentrations of Na-ASP-2 and observed for six months after the final vaccination.[51] The vaccine induced significant anti-Na-ASP-2 IgG and cellular immune responses. In addition, it was safe and produced no debilitating side effects. The vaccine is now in a phase one trial; healthy adult volunteers with documented evidence of previous infection in Brazil are being given the same dose concentration on the same schedule used in the initial study.[50] If this study is successful, the next step would be to conduct a phase two trial to assess the rate and intensity of hookworm infection among vaccinated persons. Because the Na-ASP-2 vaccine only targets the larval stage, it is critical that all subjects enrolled in the study be treated with antihelminthic drugs to eliminate adult worms prior to vaccination.

Adult hookworm antigens have also been identified as potential candidates for vaccines. When adult worms attach to the intestinal mucosa of the human host, erythrocytes are ruptured in the worm’s digestive tract which causes the release of free hemoglobin which is subsequently degraded by a proteolytic cascade. Several of these proteins that are responsible for this proteolytic cascade are also essential for the worm’s nutrition and survival.[52] Therefore, a vaccine that could induce antibodies for these antigens could interfere with the hookworm’s digestive pathway and impair the worm’s survival. Three proteins have been identified: the aspartic protease-hemoglobinase APR-1, the cysteine protease-hemoglobinase CP-2, and a glutathione S-transferase.[53][54] [55] Vaccination with APR-1 and CP-2 led to reduced host blood loss and fecal egg counts in dogs.[53][54] With APR-1, vaccination even led to reduced worm burden.[53] Research is currently stymied at the development of at least one of these antigens as a recombinant protein for testing in clinical trials.

Hookworm in therapy

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Main article: Helminthic therapy

Moderate hookworm infections have been demonstrated to have beneficial effects on hosts suffering from diseases linked to overactive immune systems. This is possibly explained by the hygiene hypothesis.[46] Research at the University of Nottingham conducted in Ethiopia observed a small subset of people with hookworm infections were half as likely to experience asthma[56] or hay fever.[57] Potential benefits have also been hypothesized in cases of multiple sclerosis,[58] Crohn's Disease[59] and diabetes.[60]

Some research conducted has shown favourable results using hookworms to treat coeliac disease.[61][62] Though research points to anti-allergenic properties associated with hook worm infections, the FDA does not currently recognize hookworms as a treatment.

See also

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References

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  1. ^ a b Fenwick A (March 2012). "The global burden of neglected tropical diseases". Public health. 126 (3): 233–6. doi:10.1016/j.puhe.2011.11.015. PMID 22325616.
  2. ^ "Neglected Tropical Diseases". cdc.gov. June 6, 2011. Retrieved 28 November 2014.
  3. ^ a b c d e f g Markell, Edward K.; John, David C.; Petri, William H. (2006). Markell and Voge's medical parasitology (9th ed.). St. Louis, Mo: Elsevier Saunders. ISBN 0-7216-4793-6.{{cite book}}: CS1 maint: multiple names: authors list (link)
  4. ^ Stoll NR (August 1962). "On endemic hookworm, where do we stand today?". Exp. Parasitol. 12 (4): 241–52. doi:10.1016/0014-4894(62)90072-3. PMID 13917420.
  5. ^ a b c d e f g h i Bethony J, Brooker S, Albonico M, Geiger SM, Loukas A, Diemert D, Hotez PJ (May 2006). "Soil-transmitted helminth infections: ascariasis, trichuriasis, and hookworm". Lancet. 367 (9521): 1521–32. doi:10.1016/S0140-6736(06)68653-4. PMID 16679166.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  6. ^ a b c d e f g h i Hotez PJ, Bethony J, Bottazzi ME, Brooker S, Buss P (March 2005). "Hookworm: "The Great Infection of Mankind"". PLoS Med. 2 (3): e67. doi:10.1371/journal.pmed.0020067. PMC 1069663. PMID 15783256.{{cite journal}}: CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
  7. ^ Hotez PJ, Pritchard DI (1995). "Hookworm infection". Scientific American. 272 (6): 68–74. doi:10.1038/scientificamerican0695-68. PMID 7761817.
  8. ^ "CDC Factsheet: Hookworm", accessed September 29, 2008
  9. ^ Hawdon JM, Hotez PJ (October 1996). "Hookworm: developmental biology of the infectious process". Curr. Opin. Genet. Dev. 6 (5): 618–23. doi:10.1016/S0959-437X(96)80092-X. PMID 8939719.
  10. ^ "Hookworms." The Center for Food Security and Public Health. May 2005. Iowa State University
  11. ^ a b c d Gasser RB, Cantacessi C, Campbell BE (January 2009). "Improved molecular diagnostic tools for human hookworms". Expert Rev. Mol. Diagn. 9 (1): 17–21. doi:10.1586/14737159.9.1.17. PMID 19099345.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  12. ^ Yong TS, Lee JH, Sim S, Lee J, Min DY, Chai JY, Eom KS, Sohn WM, Lee SH, Rim HJ (March 2007). "Differential diagnosis of Trichostrongylus and hookworm eggs via PCR using ITS-1 sequence". The Korean Journal of Parasitology. 45 (1): 69–74. doi:10.3347/kjp.2007.45.1.69. PMC 2526333. PMID 17374982.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  13. ^ "Global network for neglected tropical diseases receives $34 million from Gates Foundation: IDB leads campaign to greatly reduce the burden of most neglected diseases by 2020 in Latin America and the Caribbean." Press Release. Global Network for Neglected Tropical Diseases. 30 January 2009.
  14. ^ "Deworm the World at Clinton Global Initiative 2008 Annual Meeting: up to 10 million children to benefit from deworming!" Press Release. Deworm the World, 2008.
  15. ^ a b "School Deworming". Public Health at a Glance. World Bank. 2003.
  16. ^ "Soil-transmitted helminthiasis". Wkly Epidemiol Rec. 83 (27/28). World Health Organization: 237–252. 4 July 2008.
  17. ^ "How does deworming work?" Deworm the World. <dewormtheworld.org>
  18. ^ Massa K, Magnussen P, Sheshe A, Ntakamulenga R, Ndawi B, Olsen A (2009). "The effect of the community-directed treatment approach versus the school-based treatment approach on the prevalence and intensity of schistosomiasis and soil-transmitted helminthiasis among schoolchildren in Tanzania". Transactions of the Royal Society of Tropical Medicine and Hygiene. 103 (1): 31–37. doi:10.1016/j.trstmh.2008.07.009. PMID 18771789.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  19. ^ Howell, Daniel (2010). The Barefoot Book: 50 Great Reasons to Kick Off Your Shoes. Hunter House. ISBN 0897935543.
  20. ^ Template:Harvnb
  21. ^ Huttly SR (1990). "The impact of inadequate sanitary conditions on health in developing countries". World Health Stat Q. 43 (3): 118–26. PMID 2146815.
  22. ^ Moraes LR, Cancio JA, Cairncross S (April 2004). "Impact of drainage and sewerage on intestinal nematode infections in poor urban areas in Salvador, Brazil". Trans. R. Soc. Trop. Med. Hyg. 98 (4): 197–204. doi:10.1016/S0035-9203(03)00043-9. PMID 15049458.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  23. ^ Keiser J, Utzinger J (April 2008). "Efficacy of current drugs against soil-transmitted helminth infections: systematic review and meta-analysis". JAMA. 299 (16): 1937–48. doi:10.1001/jama.299.16.1937. PMID 18430913.
  24. ^ a b Albanese G, Venturi C, Galbiati G (2001). "Treatment of larva migrans cutanea (creeping eruption): A comparison between albendazole and traditional therapy". International Journal of Dermatology. 40 (1): 67–71. doi:10.1046/j.1365-4362.2001.01103.x. PMID 11277961.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  25. ^ Pal D, Chattopadhyay UK, Sengupta G (April 2007). "A study on the prevalence of hookworm infection in four districts of West Bengal and its linkage with anemia". Indian J Pathol Microbiol. 50 (2): 449–52. PMID 17883107.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  26. ^ Gandhi NS, Jizhang C, Khoshnood K, Fuying X, Shanwen L, Yaoruo L, Bin Z, Haechou X, Chongjin T, Yan W, Wensen W, Dungxing H, Chong C, Shuhua X, Hawdon JM, Hotez PJ (August 2001). "Epidemiology of Necator americanus hookworm infections in Xiulongkan Village, Hainan Province, China: high prevalence and intensity among middle-aged and elderly residents". J. Parasitol. 87 (4): 739–43. doi:10.1645/0022-3395(2001)087[0739:EONAHI]2.0.CO;2. PMID 11534635.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  27. ^ Verle P, Kongs A, De NV, Thieu NQ, Depraetere K, Kim HT, Dorny P (October 2003). "Prevalence of intestinal parasitic infections in northern Vietnam". Trop. Med. Int. Health. 8 (10): 961–4. doi:10.1046/j.1365-3156.2003.01123.x. PMID 14516309.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  28. ^ Fleming FM, Brooker S, Geiger SM, Caldas IR, Correa-Oliveira R, Hotez PJ, Bethony JM (January 2006). "Synergistic associations between hookworm and other helminth species in a rural community in Brazil". Trop. Med. Int. Health. 11 (1): 56–64. doi:10.1111/j.1365-3156.2005.01541.x. PMID 16398756.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  29. ^ Mabaso ML, Appleton CC, Hughes JC, Gouws E (April 2004). "Hookworm (Necator americanus) transmission in inland areas of sandy soils in KwaZulu-Natal, South Africa". Trop. Med. Int. Health. 9 (4): 471–6. doi:10.1111/j.1365-3156.2004.01216.x. PMID 15078265.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  30. ^ Brooker S, Clements AC, Bundy DA (2006). "Global epidemiology, ecology and control of soil-transmitted helminth infections". Adv. Parasitol. 62: 221–61. doi:10.1016/S0065-308X(05)62007-6. PMC 1976253. PMID 16647972.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  31. ^ Peduzzi R, Piffaretti JC (1983). "Ancylostoma duodenale and the Saint Gothard anaemia". Br Med J (Clin Res Ed). 287 (6409): 1942–5. doi:10.1136/bmj.287.6409.1942. PMC 1550193. PMID 6418279.
  32. ^ Page, Walter H. (September 1912). "The Hookworm And Civilization: The Work Of The Rockefeller Sanitary Commission In The Souther States". The World's Work: A History of Our Time. XXIV: 504–518. Retrieved 2009-07-10.
  33. ^ Bleakley H (2007). "Disease and Development: Evidence from Hookworm Eradication in the American South". Q J Econ. 122 (1): 73–117. doi:10.1162/qjec.121.1.73. PMC 3800113. PMID 24146438.
  34. ^ Birn, Anne-Emanuelle; Solórzano, Armando (November 1999). "Public health policy paradoxes: science and politics in the Rockefeller Foundation's hookworm campaign in Mexico in the 1920s". Social Science & Medicine. 49 (9): 1197–1213. doi:10.1016/S0277-9536(99)00160-4. {{cite journal}}: Invalid |ref=harv (help)
  35. ^ John A Ferrell (1914). The Rural School and Hookworm Disease.
  36. ^ a b Gyorkos TW, Larocque R, Casapia M, Gotuzzo E (September 2006). "Lack of risk of adverse birth outcomes after deworming in pregnant women". Pediatr. Infect. Dis. J. 25 (9): 791–4. doi:10.1097/01.inf.0000234068.25760.97. PMID 16940835.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  37. ^ Brooker S, Hotez PJ, Bundy DA (2008). Raso, Giovanna (ed.). "Hookworm-Related Anaemia among Pregnant Women: A Systematic Review". PLoS Negl Trop Dis. 2 (9): e291. doi:10.1371/journal.pntd.0000291. PMC 2553481. PMID 18820740.{{cite journal}}: CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
  38. ^ Larocque R, Casapia M, Gotuzzo E, MacLean JD, Soto JC, Rahme E, Gyorkos TW (October 2006). "A double-blind randomized controlled trial of antenatal mebendazole to reduce low birthweight in a hookworm-endemic area of Peru". Trop. Med. Int. Health. 11 (10): 1485–95. doi:10.1111/j.1365-3156.2006.01706.x. PMID 17002722.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  39. ^ Brooker S, Akhwale W, Pullan R, Estambale B, Clarke SE, Snow RW, Hotez PJ (December 2007). "Epidemiology of Plasmodium-Helminth co-infection in Africa: Populations at risk, potential impact on anemia and prospects for combining control". Am. J. Trop. Med. Hyg. 77 (6 Suppl): 88–98. PMC 2637949. PMID 18165479.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  40. ^ Brooker S, Clements AC, Hotez PJ, Hay SI, Tatem AJ, Bundy DA, Snow RW (2006). "The co-distribution of Plasmodium falciparum and hookworm among African schoolchildren". Malar. J. 5: 99. doi:10.1186/1475-2875-5-99. PMC 1635726. PMID 17083720.{{cite journal}}: CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
  41. ^ Spiegel A, Tall A, Raphenon G, Trape JF, Druilhe P (2003). "Increased frequency of malaria attacks in subjects co-infected by intestinal worms and Plasmodium falciparum malaria". Trans. R. Soc. Trop. Med. Hyg. 97 (2): 198–9. doi:10.1016/S0035-9203(03)90117-9. PMID 14584377.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  42. ^ Knowles SC (August 2011). "The effect of helminth co-infection on malaria in mice". International Journal of Parasitology. 15 (10): 1041–51. doi:10.1016/j.ijpara.2011.05.009. PMID 21777589.
  43. ^ Mwangi TW, Bethony JM, Brooker S (October 2006). "Malaria and helminth interactions in humans: an epidemiological viewpoint". Ann Trop Med Parasitol. 100 (7): 551–70. doi:10.1179/136485906X118468. PMC 1858631. PMID 16989681.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  44. ^ Hartgers FC, Yazdanbakhsh M (October 2006). "Co-infection of helminths and malaria: modulation of the immune responses to malaria". Parasite Immunol. 28 (10): 497–506. doi:10.1111/j.1365-3024.2006.00901.x. PMID 16965285.
  45. ^ Ruebush, Mary. Why Dirt is Good: 5 ways to make germs your friends. Kaplan, 2009.
  46. ^ a b Strachan DP (November 1989). "Hay fever, hygiene, and household size". BMJ. 299 (6710): 1259–60. doi:10.1136/bmj.299.6710.1259. PMC 1838109. PMID 2513902.
  47. ^ Cooper PJ (2004). "Intestinal worms and human allergy". Parasite Immunol. 26 (11–12): 455–67. doi:10.1111/j.0141-9838.2004.00728.x. PMID 15771681.
  48. ^ Folkerts G, Walzl G, Openshaw PJ (March 2000). "Do common childhood infections 'teach' the immune system not to be allergic?". Immunol. Today. 21 (3): 118–20. doi:10.1016/S0167-5699(00)01582-6. PMID 10777250.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  49. ^ Brode, J.E. (26 January 2009). "Babies Know: A Little Dirt Is Good for You". The New York Times.
  50. ^ a b Diemert DJ, Bethony JM, Hotez PJ (January 2008). "Hookworm vaccines". Clin. Infect. Dis. 46 (2): 282–8. doi:10.1086/524070. PMID 18171264.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  51. ^ Bethony JM, Simon G, Diemert DJ, Parenti D, Desrosiers A, Schuck S, Fujiwara R, Santiago H, Hotez PJ (May 2008). "Randomized, placebo-controlled, double-blind trial of the Na-ASP-2 hookworm vaccine in unexposed adults". Vaccine. 26 (19): 2408–17. doi:10.1016/j.vaccine.2008.02.049. PMID 18396361.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  52. ^ Williamson AL, Lecchi P, Turk BE, Choe Y, Hotez PJ, McKerrow JH, Cantley LC, Sajid M, Craik CS, Loukas A (August 2004). "A multi-enzyme cascade of hemoglobin proteolysis in the intestine of blood-feeding hookworms". J. Biol. Chem. 279 (34): 35950–7. doi:10.1074/jbc.M405842200. PMID 15199048.{{cite journal}}: CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
  53. ^ a b c Loukas A, Bethony JM, Mendez S, Fujiwara RT, Goud GN, Ranjit N, Zhan B, Jones K, Bottazzi ME, Hotez PJ (October 2005). "Vaccination with Recombinant Aspartic Hemoglobinase Reduces Parasite Load and Blood Loss after Hookworm Infection in Dogs". PLoS Med. 2 (10): e295. doi:10.1371/journal.pmed.0020295. PMC 1240050. PMID 16231975.{{cite journal}}: CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
  54. ^ a b Loukas A, Bethony JM, Williamson AL, Goud GN, Mendez S, Zhan B, Hawdon JM, Elena Bottazzi M, Brindley PJ, Hotez PJ (May 2004). "Vaccination of dogs with a recombinant cysteine protease from the intestine of canine hookworms diminishes the fecundity and growth of worms". J. Infect. Dis. 189 (10): 1952–61. doi:10.1086/386346. PMID 15122534.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  55. ^ Zhan B, Liu S, Perally S, Xue J, Fujiwara R, Brophy P, Xiao S, Liu Y, Feng J, Williamson A, Wang Y, Bueno LL, Mendez S, Goud G, Bethony JM, Hawdon JM, Loukas A, Jones K, Hotez PJ (October 2005). "Biochemical Characterization and Vaccine Potential of a Heme-Binding Glutathione Transferase from the Adult Hookworm Ancylostoma caninum". Infect. Immun. 73 (10): 6903–11. doi:10.1128/IAI.73.10.6903-6911.2005. PMC 1230892. PMID 16177370.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  56. ^ BBC Health Worm infestation 'beats asthma'
  57. ^ BBC Health Eat worms — feel better
  58. ^ Correale J, Farez M (February 2007). "Association between parasite infection and immune responses in multiple sclerosis". Ann. Neurol. 61 (2): 97–108. doi:10.1002/ana.21067. PMID 17230481.
  59. ^ Croese J, O'neil J, Masson J, Cooke S, Melrose W, Pritchard D, Speare R (January 2006). "A proof of concept study establishing Necator americanus in Crohn's patients and reservoir donors". Gut. 55 (1): 136–7. doi:10.1136/gut.2005.079129. PMC 1856386. PMID 16344586.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  60. ^ Daily Mail. The bloodsucking worm that fights allergies from inside your tummy September 14, 2007.
  61. ^ Rosendorff, D. (October 22, 2009). "Worms linked to coeliac relief". ABC News.
  62. ^ Parasites to combat celiac disease (Report). Queensland Institute of Medical Research. April 3, 2008.

Notes

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  •   This article incorporates text from a publication now in the public domain: Chisholm, Hugh, ed. (1911). "article name needed". Encyclopædia Britannica (11th ed.). Cambridge University Press. {{cite encyclopedia}}: Cite has empty unknown parameters: |HIDE_PARAMETER15=, |HIDE_PARAMETER13=, |HIDE_PARAMETER14c=, |HIDE_PARAMETER14ab=, |HIDE_PARAMETER3=, |HIDE_PARAMETER1=, |HIDE_PARAMETER4=, |HIDE_PARAMETER2=, |HIDE_PARAMETER8=, |HIDE_PARAMETER14bb=, |HIDE_PARAMETER20=, |HIDE_PARAMETER5=, |separator=, |HIDE_PARAMETER14b=, |HIDE_PARAMETER14cb=, |HIDE_PARAMETER14a=, |HIDE_PARAMETER10=, |HIDE_PARAMETER9=, |HIDE_PARAMETER6=, |HIDE_PARAMETER7=, |HIDE_PARAMETER11=, and |HIDE_PARAMETER12= (help); Invalid |ref=harv (help)
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